Immunohistochemistry demarcating M2 like macrophage populations in human gastrocnemius muscle. 592:2625-2636.įiber Type Specific Pax7 Staining on Human Muscle Cryosectionsįiber Type Specific Pax7 Staining on Mouse Muscle Cryosections Fiber type-specific satellite cell response to aerobic training in sedentary adults. Anti-laminin is used to delineate fiber borders and type 1 myosin heavy chain (MyHC) antibody to determine fiber type, so that satellite cells can be assigned to specific fiber types.įry, C.S., B. Immunohistochemical analysis of satellite cells (SCs, Pax7+) in human vastus lateralis muscle.
Elevated myonuclear density during skeletal muscle hypertrophy in response to training is reversed during detraining. Mouse skeletal muscle expresses 4 MyHCs, including 2b, which can be quantified using a modified immunohistochemistry protocol.ĭungan, C.M., K.A. Osteoarthritis and Cartilage.įiber Type Staining on Human Muscle Cryosections Alterations in quadriceps muscle cellular and molecular properties in adults with moderate knee osteoarthritis. Noehren B, Kosmac K, Walton RG, Murach KA, Lyles MF, Loeser RF, Peterson CA and Messier SP. Peterson (2014). Fiber type-specific satellite cell response to aerobic training in sedentary adults. Hybrid 2a/2x fibers are more commonly observed, whereas pure 2x fibers are also rare.įry, C.S., B. MyHC 1/2a fibers are present but rare, typically comprising less than 5% of total fibers in a biopsy. Hybrid fibers, composed of more than one MyHC, can also be found in human skeletal muscle. Fiber type composition is one factor underlying the contractile and metabolic properties of a muscle. Human skeletal muscle is composed of three myosin heavy chain (MyHC) isoforms: 1, 2a and 2x, identified by isoform-specific antibodies. Using anti-laminin to label fiber borders, total and fiber type specific cross sectional area can be analyzed. Immunohistochemical analysis of fiber type distribution in the vastus lateralis muscle of a patient with osteoarthritis. We are proud to support basic, clinical and translation research aimed at understanding the mechanisms underlying the regulation of muscle structure and function and the impact on physical activity and chronic disease. If you have questions or would like to schedule a consultation or reserve time on MIMIC equipment (listed below), please contact us. MIMIC provides access to automated image analyses software Myovision for download, and will provide high throughput image quantification upon request. Kate Kosmac, offers expertise, training and protocols for researchers working with muscle, including sectioning of frozen muscle tissue, histological and immunohistochemical muscle characterization and imaging of common muscle features. The Muscle Immunohistochemistry and Molecular Imaging Core (MIMIC), directed by Dr.
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